T2P Illumina Library Preparations

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All the Illumina Libraries on this Page are Prepared with our T2Prep System.

We now produce a variety of Illumina libraries using NEB chemistry with minor only modifications to the base protocol in our closed tube T2Prep system. These libraries are excellent for service production because of the simplicity of the methods, robust chemistry with low or high inputs amounts, and they provide us with a very stable adapter chemistry for use with robotics. These preps were some of the first sets of chemistry we tested in our system. We also were able to expand the indexing capabilities with this chemistry to suit the needs of varying sizes of projects and sequence coverage needs. Recently, we have modified the chemistry to improve results on HiSeq4000, specifically to prevent abnormally high indexing swapping often associated with dual indexed samples from non-Illumina preparations, ensuring high levels of identified indices using either Single or Dual indexing formats. These libraries are very high quality and offer excellent consistency as verified by our production controls. Please visit our HiSeq4000 sequencing page and review the yields for determining what type of sequencing you prefer. The primary reason we only offer limited runs is so that we continue to fill flowcells quickly and ensure you receive data in 21 days or less. If you have a lot of samples to get sequenced these libraries are our most economical option. Please contact us for questions before ordering.

  • 5-7 day Turnaround time for libraries on any sized project.
  • Full QC reports on every sample, including free pre- and post-library QC.
  • Our T2Prep system ensures pure samples and high index call rates.
  • No charge replicates and batch controls for monitoring quality and dynamic range.
  • Combine these libraries, with any indexing combination, and our ExAmp compatible purification scheme for HiSeq4000 to achieve true production level datasets in 21 days or less for tens to several hundreds of libraries.
  • Verifiable quality and scale.

Description / Library Features

Since we take the standard SBS format and create a closed tube system through several consumables innovations, we can process projects faster, standardize quality, and price libraries extremely competitively.

Based on the size of your project, samples can be processed quickly in a Low or High Throughput format.

Low Throughput

High Throughput

Batch Controls, Process Controls, and Spike-Ins:

Each Order receives free of charge controls if 8 or more libraries are being created, ensuring all libraries meet stringent application specific specifications.

Final Library Specifications

Select Molecular Preps for your Order:

  • 3′ RNAseq ($/sample for 1-24 preps)

    $ 100.00
  • 3′ RNAseq ($/sample for 25 preps)

    $ 55.00
  • Genome Skim DNA Library ($/sample for 1-24 preps)

    $ 80.00
  • Genome Skim DNA Library ($/sample for 25 preps)

    $ 40.00
  • RADseq/GBS ($/sample for 1-24 preps)

    $ 50.00
  • RADseq/GBS ($/sample for 25 preps)

    $ 25.00
  • RNAseq Library ($/sample for 1-24 preps)

    $ 80.00
  • RNAseq Library ($/sample for 25 preps)

    $ 55.00
  • Whole Genome ($/sample for 1-24 preps)

    $ 80.00
  • Whole Genome ($/sample for 25 preps)

    $ 40.00

Interested in customizing certain aspects of these popular library types, or have questions about your study design? 

No problem, use the contact form to request info.

Click here to browse our entire service portfolio.