Custom & Legacy Services

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Global Biologics has worked for several years with a variety of NGS library protocols deployed across a variety of different species and projects. As many of these services became obsolete, inefficient, infrequently ordered, or adversely expensive; we have naturally removed them from our online catalog or replaced them with current versions. We refer to these as legacy services, many of which are still available on a custom project basis. Using the contact form provided, please communicate what services you would like performed and we will reply with pricing and availability.  A short list of legacy services which were very commonly utilized is provided for reference.

Service Description
DNase I Trtmnt+QC DNaseI treatment of total RNA, followed by Cleanup, Fragment Analysis and Quantitation
RNase Trtmnt+QC RNase treatment of total RNA, followed by Cleanup, Fragment Analysis and Quantitation
qPCR validation,quant, pooling Post prep Fragment Analysis, qPCR quantitation using Kapa Bio (Illumina) kit, 4-10nM dilution and mutliplex pooling of GB generated libraries.
UltraLow-SMRTv4 UltraLow input RNA processing via Clontech/Takara SMRTv4 cDNA Kit, with Advantage 2 LongeRange PCR Polymerase
16S Metagenomics + MiSeq 1 – 16S V1-V3(V1-V3 F28)(V1-V3 R519)
16S Metagenomics + MiSeq 2 – 16S V3-V5(V3-V5 F357)(V3-V5 R926)
16S Metagenomics + MiSeq 3 – 16S V4(V4 515F)(V4 806R)
16S Metagenomics + MiSeq 4 – Archaea(Arch349F)(Arch806R)
16S Metagenomics + MiSeq 5 – Eukaryotic 18S(F566Euk)(R1200Euk)
16S Metagenomics + MiSeq 6 – Eukaryotic 18S(Euk_1391F)(EukBr-7R)
16S Metagenomics + MiSeq 7 – ITS1-ITS4(ITS1F)(ITS4R)
16S Metagenomics + MiSeq 8 – ITS3-ITS4(ITS3F)(ITS4R)
ALLPATHS Library Prep Illumina Library Preparations: 180bp Insert (Small Insert), 3kb and 8kb insert Nextera Mate Pair (Large Inserts), Pooled for sequencing at ~50% small insert, ~37.5% 3kb large insert, and ~12.5% 8kb large insert.
ChIP-Seq Library Prep & QC Illumina  Preparation with 48 barcodes available and Pippin Prep Gel Selection. Price includes pre-prep and post-prep (DNA) QC.
MeMiner CpG Enrichment Methylation Enrichment procedure: methylated DNA is isolated from fragmented whole genomic DNA (5 ng–25 µg) via binding to the methyl-CpG binding domain of human MBD2 protein, which is coupled to paramagnetic Dynabeads M-280 Streptavidin via a biotin linker. The methylated fragments can then be eluted as a single enriched population for microarray or sequencing.
MiSeq Library Validation Run Validation of library using MiSeq, with data provided for low coverage alignment and library concentration information.
NEBNext® FFPE DNA Repair Mix Damaged DNA Repair: Archiving of clinical materials as Formalin-Fixed, Paraffin-Embedded (FFPE) samples is a common practice. However, the methods used for fixation and storage significantly damage and compromise the quality of nucleic acids from these samples. As a result, it can be challenging to obtain useful information, including high-quality sequence data, especially when sample amounts are limited. The NEBNext FFPE DNA Repair Mix is a cocktail of enzymes formulated to repair DNA, and specifically optimized and validated for repair of FFPE DNA samples. Will repair: Deamination of cytosine to uracil, Nicks and gaps, Oxidized bases, Blocked 3´ ends; Will NOT repair: DNA fragmentation, DNA-protein crosslinks. This approach does show some improvement of library diversity and yields with mammalian museum specimens and in some cases aged plant DNA samples.
Ribo-Minus (Bacteria) Reduction of ribosomal RNA from gram negative or gram positive bacteria using LifeTech’s RiboMinus Kit
Ribo-Minus (Eukaryote) Reduction of ribosomal RNA from eukaryote samples using LifeTech’s RiboMinus Kit
Ribo-Zero (Bacteria) Reduction of ribosomal RNA from gram negative or gram positive bacteria using Illumina’s RiboZero Kit
Ribo-Zero (Plant) Removes cytoplasmic, mitochondrial and chloroplast ribosomal RNA in a single pass. Good for intact and partially degraded small samples. The sequencing data from samples treated with Ribo-Zero (Plant Leaf) and Ribo-Zero (Plant Seed/Root) is focused on valuable RNA species leading to discovery of relevant genes, splice variants, and isoforms. Ribo-Zero (Plant Leaf) and Ribo-Zero (Plant Seed/Root) offer the most informative sequencing results by removing unwanted cytoplasmic, mitochondrial, and chloroplast ribosomal RNA prior sequencing. In order to achieve maximum depletion of rRNA, the Ribo-Zero (Plant Leaf) kit contains a higher percentage of probes to deplete chloroplast rRNA compared to Ribo-Zero (Plant Seed/Root)
Ribo-Zero Gold (H/R/M) Ribo-Zero™ Gold Kit (Human/Mouse/Rat) removes both cytoplasmic (nuclear-encoded) rRNA and mitochondrial rRNA from 1 to 5 µg of human, mouse, or rat total RNA preparations.
ERCC Ex-Fold spike in External RNA Controls Consortium (ERCC) Ex-Fold Spike-In Control for Gene Expression Sequencing (RNAseq). Life Tech Part #4456739
Bioinformatics-miRNA-Profiling miRNA-Seq Profiling and Bioinformatics processing includes data cleaning/trim, alignment and expression analysis using miRbase reference. Does include novel transcript discovery report, graphical data assessments and test statistics. Does NOT include functional classification and metabolic pathway analysis
Bioinformatics-miRNA-Profiling+ miRNA-Seq Profiling and Bioinformatics processing includes data cleaning/trim, alignment and expression analysis using miRbase reference. Does include novel transcript discovery report, graphical data assessments and test statistics, also includes functional classification of the top targets and putative metabolic pathway analysis.
Bioinformatics-RNAseq-Assembly mRNA-Seq Profiling and Transcriptome characterization and assembly. Processing includes data cleaning/trim, alignment and/or assembly, gene expression analysis using Cufflinks,Cuffdiff,DEseq pipeline and novel transcript discovery reports with graphical data assessments and test statistics. Functional gene classification and metabolic pathway analyses of gene products will be provided.
Bioinformatics-RNAseq-Profiling mRNA-Seq Profiling and Bioinformatics processing includes data cleaning/trim, alignment and gene expression analysis using Cufflinks,Cuffdiff,DEseq pipeline. Does include novel transcript discovery report, graphical data assessments and test statistics. Does NOT include functional gene classification and metabolic pathway analysis of gene products or full transcriptome assembly.
Bioinformatics-SNP ID Bioinformatics Processing-SNP: Flat fee for processing raw NGS data through to high coverage SNP list
Illumina PCRfree Genome Library Illumina PCRfree Genome Library Preparation: Ideal for whole genome assembly & Resequencing, includes Covaris shearing and qPCR quantitation of the library prior to Illumina sequencing, 300-400bp insert. Minimum order of 6
PaxGene Total RNA Isolation Use of PaxGene miRNA+Total RNA Kit
Mate Pair Library (Illumina) Illumina Library Preparations: 2-12kb, 3kb, or 8kb insert sizes created using Illumina Nextera Mate Pair Kit and Sage Pippin Prep system
HiSeq 2500 v3 HiSeq2500 v3 SBS chemistry: 100, 150 & 250bp read lengths, single or paired end, single or dual indexes, Rapid (2lanes) or HiOutput (8lanes) modes
NextSeq500 NextSeq500 chemistry. 75 & 150 bp read lengths, single or paired end, single or dual indexes, Standard or HiOutput modes

Project information:

Grant PurposeBid OpportunityActively Funded Project

Consultation

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