DEDICATED ILLUMINA SERVICES

High Performance Genome Sequencing

Scalable Offering Delivered Fast.

Welcome!

Q: Are you looking for sequencing data to be generated quickly so that downstream bioinformatics and validation efforts can proceed efficiently?

Q: Are you on a tight timeline to publish your results?

Q: Do you require the expertise that enables quality results for processing for tens, hundreds, or even thousands of samples in a relatively short time-frame?

A: Look no further! We are experts in Illumina sequencing and have worked for years to develop a scalable processing pipeline that empowers individual researchers & teams to generate affordable, high quality whole genome and expression sequencing data for researchers.

 


 

We’ve fine tuned every step of the NGS process incorporating time savings and quality assurance steps which yield affordable and high quality Illumina data in 7-10 days.

 

–>Our process is extremely scalable. No project is too large or small. All our assays utilize ~50ng of input RNA or DNA.

–>We have a unified pricing model. Our sequencing services are affordable to you and easy for us to scale and manage.

–>Our sequencing capability is redundant. With the support of our key partners (who are industry leaders), we offer a variety of RNA, DNA, skimming, capture & genotyping applications. Our format assures samples are processed quickly and uniformly, and to provide data on time and per specification.

 

How To Order

 

1. Review our Services page to see what we offer in general terms. Or Build a quotation to get immediate pricing or login to see Rapid service pricing and descriptions.

 

2. Review our Illumina Sequencing Schedule & Decide which week you would like to submit samples and when data will be returned. We offer 3 day or 7 day rapid services for RNAseq, WGS, miRNA, and UHT library projects.

 

3. Build a Quotation or Contact Us to get advice about your project and reserve a spot in our queue.

 

4. Confirm your order details and sample readiness to initiate the project. Within 24 hours you will receive a FedEx Overnight shipping label to deliver your samples to our lab (free of charge shipping label, just add dry ice and drop at the nearest FedEx shipping center).

 

5. We will process Samples according to your request and post your data to our repository. Once complete, you must approve results were successfully delivered and then will be requested to pay the bill.

 

Discounts & Promotions: from time to time we run promotions and offer discounts for referrals and volume Clients. When you create a quotation/order request, you will be prompted to enter the discount code and your discount will automatically be applied to your next order.

 

Service Features

Key Features of Our Process:

    • Custom-developed 15-minute sample quantitation & normalization process.

    • A 2-hour library preparation process powered by high-performance library technology (described in your project reports, or inquire with questions), developed by major US vendors and validated at reputable Genome Centers, including our laboratory.

    • A custom 90-minute post library quality control process which validates, measures concentration and normalizes samples quickly, resulting in evenly pooled libraries for your sequencing project.

    • Balanced read outputs: 95% of your libraries will yield guaranteed minimum read counts with <20% CV to assure even coverage for your project. Samples with too few reads will QC’d again and additional sequencing generated with 7 days to satisfy order criteria.

    • Sequencing runs are pre-scheduled & fully paid for in advance, both in our lab and at partnering labs that help us maintain flexibility and speed. We utilize a closed tube prep process that ensures pure libraries and are very confident our samples will sequencing optimally every time. This allows us to accommodate projects of any size. We can process small and large projects with equal efficiency and without scheduling delays associated with obtaining samples to fill flowcell lanes from a limited user base.

    • Fast, redundant and secure HIPAA compliant LIMS tracking, data repository and distribution process.

    • Setup a web account on our website & Login to view pricing for additional services including Small RNAseq, dd/3d RADseq, and customized sequencing outputs for various libraries to be ordered on a contract basis.

    • Highly parallel Bioinformatics available. Next day processing including automated report generation and interactive publication quality visualization, powered by an industry leading partner in Sequencing Bioinformatics.

Illumina Service Schedule
Illumina Seq. Schedule
(Submit 100-200ng of Total RNA or gDNA to Begin)
Submit Sample Seq. Run Date Data Release
Feb 4-7 Feb 10 & 12 Feb 12 & 14
Feb 11-14 Feb 17 & 19 Feb 19 & 21
Feb 18-21 Feb 24 & 26 Feb 26 & 28
Mar 3-6 Mar 9 & 11 Mar 11 & 13
Mar 10-13 Mar 16 & 18 Mar 18 & 20
Mar 17-20 Mar 23 & 25 Mar 25 & 27
Mar 31-Apr 3 Apr 6 & 8 Apr 8 & 10
Apr 7-10 Apr 13 & 15 Apr 15 & 17
Apr 14-17 Apr 20 & 22 Apr 22 & 24
Apr 21-24 Apr 27 & 29 Apr 29 & Mar 1
Services

Processing Modes: All our Illumina services are available in three Formats (or modes of processing):

  1. Rapid 3 Day: Libraries are prepared the same day we receive and sequenced the following day. Results are posted on the third business day.

  2. Rapid 7 Day: Libraries are prepared in 1-2 business days and sequenced within 1-2 business days. Results are posted on the 7th business day.

  3. Standard 30 Day Turnaround: This service is for users who require targeted capture or species specific processing. Projects are quoted individually based on volume, submission date, and chemistry required to complete the project per user qualifications.

Library Preparations: we stock the following chemistry for Illumina library construction, all of which are sensitive with as little as 1 ng of input material.

  1. RNAseq: NEB Ultra II Directional RNAseq (dT) or Zymo Total RNAseq (rRNA any species)

  2. DNAseq / WGS / Skimming / Capture Preparations: SeqOnce – RhinoSeq, iGenomix-RipTide, NEB UltraII

  3. Small RNA: Somagenics-Realseq

 

Sequencing: we use the following Illumina platforms:

  • MiSeq: Library Pool Validation Prior to High Output Sequencing

  • NextSeq550: For small projects, we run samples on NextSeq due to the single flowcell format and rapid run time.

  • NovaSeq6000: For large projects, we run samples on NovaSeq to ensure rapid completion and high outputs. NovaSeq also offers fast run times & the lowest price per Gb of data.

  • We use unique dual indexing on all projects to ensure proper sample identification (ie demultiplexing).

  • All data will meet or exceed Illumina quality specifications for the sequencing run utilized. In general, this mean 75-80% of all bases at Q30 at 150bp (and better for shorter read runs). Illumina spec sheets available upon request.

 

Bioinformatics:

  • All runs are processed through the standard Illumina purity filtering and demultiplexing scripts which result in fastq output for each sample (based on index).

  • We run base level quality control to ensure unbiased sequencing reads are distributed with no contamination.

  • We offer high level bioinformatics using a scalable pipeline through our partner. This includes full alignments, statistical analyses, feature calling, and publication quality plots which are available for viewing and download online. Data is processed directly from our data repository in a highly parallel fashion, and made available the next business day. No coding delays, no script conflicts and no wondering when results will be ready.

Sample Requirements:

Library Type Amount Volume (ul)
Total RNA for RNAseq 0.25-1.5ug 10
Total RNA for Small RNA seq 0.25-1.5ug 10
Genomic DNA-Std Input, High Quality/mw 0.25-1.5ug 10
Genomic DNA-Low Input/Quality/Aged 0.05-0.25ug 10
*Please concentrate or dilute samples so that you can provide the appropriate amount

 


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